This course aims to help students develop knowledge in the field of natural material extraction and isolation technology, starting from the process of selecting and preparing natural materials, considerations in selecting the type of extraction and isolation technique to evaluating the results in the form of products with high economic value.

• Compare various principles related to extraction technology and the isolation of natural materials.
• Determine appropriate extraction and/or isolation techniques for specific natural materials.
• Develop process skills to solve problems related to extraction technology and natural material isolation.

• Natural Substances and Secondary Metabolites

  • Introduction to natural substances and their importance in pharmaceuticals and industries
  • Overview of secondary metabolites and their roles in plants and microorganisms

• Variety of Natural Material Products

  • Classification and examples of natural material products (e.g., essential oils, alkaloids, flavonoids, terpenes)
  • Applications in food, medicine, cosmetics, and other industries

• Selection and Preparation of Materials for Extraction

  • Criteria for selecting natural materials based on desired compounds
  • Preparation techniques (e.g., drying, grinding, and maceration) for effective extraction

• Selection and Preparation of Materials for Isolation

  • Methods for isolating active compounds from natural sources
  • Considerations for ensuring purity and quality during preparation

• Natural Material Extraction Techniques

  • Overview of extraction methods:
    • Solvent extraction (liquid-liquid and solid-liquid)
    • Steam distillation
    • Supercritical fluid extraction (SFE)
  • Selection of appropriate extraction techniques based on material properties

• Fractionation Techniques and Isolation of Natural Materials

  • Techniques for fractionating natural extracts:
    • Chromatography (e.g., column, thin-layer, gas chromatography)
    • Precipitation and filtration
  • Methods for isolating target compounds from complex mixtures

1. Rydberg, Cox, & Musikas, Solvent Extraction Principles and Practice, 2nd ed., Marcel Dekker, Inc., 2004.
2. Meireles, M. Angela A., Extracting Bioactive Compounds for Food Products: Theory and Applications, CRC Press – Taylor & Francis Group, LLC, 2009.
3. Rostagno, Mauricio A., & Prado, Juliana M., Natural Product Extraction: Principles and Applications, RSC Publishing, 2013.