This course is an elective course related to the sub competence of being able to determine the concepts and techniques of genetic and protein engineering and apply them to cloning and protein production. This course discusses the concepts and techniques of protein engineering for various interests in the pharmaceutical, medical, food and other biotechnology industries. The scope of the material is about protein purification techniques, protein engineering strategies for separation, biocatalysis, and medical purposes, and their applications in related industries. By taking this course, students are required to determine protein engineering strategies for protein production.

• Determine protein engineering strategies for applications in separation, biocatalysis, and medicine.

• Introduction

  • Overview of protein engineering and its applications in biotechnology
  • Importance of protein design, modification, and optimization

• Protein Docking Methods

  • Fundamentals of protein-ligand docking techniques
  • Software tools and methods for protein docking simulations

• Protein Tagging Strategies

  • Techniques for attaching tags to proteins for purification and detection
  • Common protein tagging methods (e.g., His-tag, GFP, biotin)

• Gene Synthesis Design

  • Principles of designing genes for protein expression
  • Tools and techniques for synthesizing custom genes

• Enzyme Stabilization

  • Methods for enhancing enzyme stability and activity
  • Applications in industrial and pharmaceutical processes

• Molecular Exploration

  • Approaches to studying protein structure and function
  • Use of computational methods and molecular dynamics simulations

• Protein Engineering

  • Techniques for designing and modifying proteins with desired properties
  • Applications in enzyme engineering, therapeutic proteins, and biosensors

• Case Study

  • Real-world applications and case studies in protein engineering
  • Discussion of successful projects and challenges in the field

1. Lilia Alberghina, Protein Engineering in Industrial Biotechnology, Harwood Academic Publishers, 2005.
2. Dr. Gary Walsh, Proteins: Biotechnology and Biochemistry, Wiley.
3. Sheldon J. Park & Jennifer R. Cochran, Protein Engineering and Design, CRC Press.
4. Paul R. Carey, Protein Engineering and Design, Academic Press.
5. Protein Engineering: Principles and Practice, Wiley-Liss.